Fig. 7.

Loss of ampk function up-regulates Yki target genes. (A and B) Protein levels of Kib and Ex are increased in hsFLP/FRT-generated ampk³ clones. Clones are marked by the absence of GFP expression. (C–F) RNAi against ampk rescues inhibition of Yki target gene expression by LKB1. LKB1 overexpression in the optix compartment (D) reduces DIAP1 protein levels in comparison with the control (C). The caspase inhibitor p35 was coexpressed to prevent cell death upon LKB1 over-expression in (C and D). (E) Expression of UAS-ampk RNAi^GD736 alone does not modify DIAP1 levels. (F) Expression of UAS-ampk RNAi^GD736 together with UAS-lkb1 rescues the reduction of DIAP1 levels. The optix compartment is marked by GFP expression in C–E. (Scale bars, 50 μm.)