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. 2015 Aug 31;112(37):E5169–E5178. doi: 10.1073/pnas.1505512112

Fig. S2.

Fig. S2.

LKB1 inhibits Yki activity downstream of Wts. (A and B) LKB1 overexpression inhibits Yki target gene expression in the presence of YkiS111A S168A S250A (Yki3SA). (A) Overexpression of Yki3SA alone in the optix compartment does not modify protein levels of Yki target gene Kib. (B) Expression of LKB1 together with Yki3SA in the optix compartment reduces Kib protein levels. The optix compartment is marked by GFP expression. (C and D) LKB1 overexpression is not sufficient to restore Kib levels in Yki3SA-expressing MARCM clones in the optic lobe to wild-type levels. (E) Protein levels of the Yki target Ex are increased in wtsx1 clones generated with MARCM system in the NE of third instar larval brains. (F) Overexpression of LKB1 in wtsx1 clones reduces Ex levels caused by wts loss. Clones are marked by GFP expression. (Scale bars, 20 μm in A and B and 50 μm in C and F.) (G) Quantification of Yki3SA-V5 localization in NBs of MARCM clones of the indicated genotypes in third instar larval CB. C, cytoplasmic Yki; N/C, cytoplasmic and nuclear Yki. n = 67 (FRT82B), 51 (lkb1x5).