Fig. 6.

ARF18 expression patterns by RT-qPCR and GUS assays. (A) Analysis of ARF18 expression in selected tissues in R1 using RT-qPCR. ENTH was used as the reference. Data are expressed as the means of three biological replicates; error bars indicate SDs. (B) GUS expression patterns(blue staining) at various stages in the ARF18 promoter-GUS transgenic line. GUS was strongly expressed in the cotyledon (cn), seedling (sl), older leaves (lf), and silique wall (sw). Moderate expression levels were detected in the bud (bd) and embryo (sd). No expression was observed in the seed coat (sd). (Scale bars, 5 mm for cotyledon, seedling, and older leaves; 0.8 mm for silique wall and bud; 0.1 mm for embryo.)