Table 1.
pHi, aNai, aCli, and Vm measurements in Nha1, Nha2, and control oocytes
Condition | Units | Nha1 | Nha2 | Control | |||
Average ± SEM | n | Average ± SEM | n | Average ± SEM | n | ||
Resting pHi (96Na, 103.6Cl, pH 7.5) | 7.16 ± 0.02*** | 31 | 7.19 ± 0.06 | 6 | 7.33 ± 0.04 | 24 | |
ΔpHi/dt (96Na, 103.6Cl, pH 7.5) | × 105 pH units/s | −0.4 ± 0.6 | 30 | −2.5 ± 1.6 | 18 | −1.7 ± 0.7 | 6 |
ΔpHi/dt (96Na, 103.6Cl, pH 8.5) | × 105 pH units/s | 51.0 ± 6.1** | 3 | 46.0 ± 6.6** | 3 | 3.3 ± 1.3 | 3 |
ΔpHi/dt (0Na, 103.6Cl, pH 7.5) | × 105 pH units/s | 1.0 ± 7.9 | 3 | −51.0 ± 9.1** | 3 | −3.7 ± 1.7 | 3 |
ΔpHi/dt (96Na, 0Cl, pH 7.5) | × 105 pH units/s | 120 ± 16*** | 37 | −2.0 ± 0.0 | 7 | 3.0 ± 1.8 | 10 |
ΔpHi/dt (0Na, 0Cl, pH 7.5) | × 105 pH units/s | 167 ± 17*** | 14 | −9.8 ± 2.2 | 4 | ||
Resting aNai (96Na, 103.6Cl, pH 7.5) | mM | 3.7 ± 0.3*** | 6 | 4.4 ± 0.6*** | 11 | 1.8 ± 0.1 | 7 |
ΔaNai/dt (96Na, 103.6Cl, pH 7.5) | mM/min | 0.005 ± 0.003 | 12 | −0.004 ± 0.003 | 10 | 0.002 ± 0.002 | 6 |
ΔaNai/dt (96Na, 103.6Cl, pH 8.5) | mM/min | −0.001 ± 0.003 | 3 | 0.029 ± 0.002** | 3 | 0.009 ± 0.005 | 4 |
ΔaNai/dt (0Na, 103.6Cl, pH 7.5) | mM/min | −0.014 ± 0.004 | 3 | −0.055 ± 0.006*** | 8 | −0.018 ± 0.003 | 7 |
ΔaNai/dt (96Na, 0Cl, pH 7.5) | mM/min | 0.060 ± 0.008*** | 8 | 0.002 ± 0.003 | 3 | −0.002 ± 0.002 | 6 |
ΔaNai/dt (0Na, 0Cl, pH 7.5) | mM/min | −0.001 ± 0.002 | 5 | −0.009 ± 0.001 | 3 | ||
Resting aCli (96Na, 103.6Cl, pH 7.5) | mM | 51.0 ± 3.5* | 15 | 42.5 ± 2.1 | 13 | 41.7 ± 1.7 | 8 |
ΔaCli/dt (96Na, 103.6Cl, pH 7.5) | mM/min | −0.04 ± 0.04 | 6 | −0.01 ± 0.04 | 7 | −0.07 ± 0.03 | 7 |
ΔaCli/dt (96Na, 103.6Cl, pH 8.5) | mM/min | −0.74 ± 0.09** | 4 | 0.00 ± 0.06 | 3 | −0.11 ± 0.08 | 3 |
ΔaCli/dt (0Na, 103.6Cl, pH 7.5) | mM/min | −0.18 ± 0.03 | 3 | −0.14 ± 0.10 | 3 | −0.04 ± 0.04 | 3 |
ΔaCli/dt (96Na, 0Cl, pH 7.5) | mM/min | −1.85 ± 0.39*** | 7 | −0.12 ± 0.05 | 7 | −0.06 ± 0.02 | 4 |
Resting Vm (96Na, 103.6Cl, pH 7.5) | mV | −37.0 ± 1.9*** | 52 | −35.1 ± 2.2*** | 48 | −49.7 ± 1.8 | 22 |
ΔVm (96Na, 103.6Cl, pH 8.5) | mV | −2.2 ± 1.4 | 9 | 0.9 ± 0.6 | 9 | −4.0 ± 1.6 | 10 |
ΔVm (0Na, 103.6Cl, pH 7.5) | mV | −18.5 ± 1.5*** | 9 | −12.7 ± 1.7 | 9 | −7.2 ± 2.3 | 10 |
ΔVm (96Na, 0Cl, pH 7.5) | mV | 24.8 ± 2.7*** | 30 | 4.1 ± 1.6 | 13 | −0.3 ± 1.6 | 9 |
n, number of experiments. Statistical significance was calculated by Student's t test. ***P < 0.001; **P < 0.01; *P < 0.05.