Fig. 6.

Altered lipid metabolism in Tfr1^IEC-KO IECs. (A) Electron micrographs showed large inclusions in IECs of Tfr1^IEC-KO intestinal epithelium and similar, smaller inclusions in Tfr1^fl/fl epithelium at P1 (white arrows). (Scale bars, 10 μM.) (B) Oil-red-O staining of P1 intestinal epithelium confirming that inclusions, seen as large red spots in Tfr1^IEC-KO IECs, contain neutral lipids. Staining was more diffuse in Tfr1^fl/fl IECs. (Scale bars, 100 μM.) See Fig. S4 for additional detail. (C) Heat map of genes reported to be down-regulated in Plagl2^−/− mice (28), from microarray profiles of Tfr1^fl/fl, Tfr1^IEC-KO, and Tfr1^IEC-KO;Rosa26-Tfr1^R654A/+ IECs.