Table S2.

Kinetic data for displacement of Sp1 ZF-DBD by Egr-1 ZF-DBDs

Egr-1 ZF-DBD	kdisp (s−1)*	kdisp/koff,Sp1†	kdisp/Aen, M−1 s−1‡
Type 1	0.17 ± 0.04	15 ± 4	(0.7 ± 0.1) × 106
Type 0	0.09 ± 0.01	9 ± 1	(1.4 ± 0.3) × 106
Type 2	0.13 ± 0.01	12 ± 1	(2.2 ± 0.2) × 106
Type 3	0.18 ± 0.01	16 ± 2	(3.2 ± 0.3) × 106

^*Values of the first-order rate constant for displacement k_disp were obtained from the data shown in Fig. 5B via nonlinear least-squares fitting with k_obs = k_disp P_tot/(A_en + P_tot), where P_tot represents a total concentration of Egr-1 ZF-DBD, and A_en represents an apparent steady-state constant for the encounter complex.

^†The parameter k_off,Sp1 represents the rate constant for Sp1 ZF-DBD’s spontaneous dissociation from the overlapping target site (Fig. S6). The ratio k_disp/k_off,Sp1 corresponds to enhancement of Sp1 ZF-DBD’s dissociation by Egr-1 ZF-DBD.

^‡This corresponds to the second-order rate constant for the overall process at relatively low concentrations of Egr-1 ZF-DBD (i.e., P_tot << A_en).