Fig. 2.
Translocation events in the absence of XerCD. (A) Translocation event. (Top) Intensity: green emission under green excitation (DD, green), red emission under green excitation (DA, red), and red emission under red excitation (AA, black). (Middle) Apparent FRET. Under these conditions, no FRET was observed. (Bottom) FIW in the green channel (green) and in the red channel (black), and LS fit to the red FIW (red) used to segment the event into before (purple shading), during (green shading), and after (orange shading) translocation (n = 179 events). (B) Start and end positions of translocations. Black arrows represent positions of features on the DNA, obtained using the FIW calibration. (C) Dwell times. (Top) FtsKC assembly. (Bottom) Residence after translocation. Distributions were fit using a two-exponential process (Table S1 and Methods).