Fig. 5.
Arrival of FtsKC at XerCD-dif synapses. (A) FtsKC arrives after synapsis formation, leading to recombination. (Inset) Successful recombination reactions result in a permanent narrow green FIW. The highlighted region corresponds to the main figure. FtsKC binding is apparent after synapsis formation (but not during synapse formation) in 39 of 73 recombination events that meet the 90% confidence criterion. (B) FtsKC arrives at a synapsis and dissociates before the synapsis disassembles. (Inset) Synapsis formation and disassembly are apparent as a transiently narrowing FIW (n = 48, of which 20 show FRET). (C) Start and end positions of translocations. The mean starting FIW is 221 ± 4 nm (±SEM), and the mean end FIW is 175 ± 2 nm (±SEM). (D) Dwell times before and after translocation. Distributions are fit using MATLAB (Table S1).