Fig. S9.

FRET reports on the proximity of FtsK_C to the XerCD bound to dif. The dwell time distribution for automatically extracted FRET events (lasting a minimum of two frames) for unsynapsed (Top) and product (Bottom) DNA is fit using two exponentials (Table S1). The dwell times during and after FRET are similar for both DNA conformations, and the short time between the end of FRET and the disappearance of red fluorescence suggests that FtsK_C dissociates after meeting XerCD bound to dif, rather than reversing translocation direction.