Fig. S2.

Quantification of CXCR4 sequestration in the cytoplasmic compartment upon ERK1/2 activation. Intensity plots derived from 20 confocal fluorescence microscopy images obtained in three independent experiments (illustrated in Fig. 3) were analyzed to quantify CXCR4 localization at the cell membrane and inside the cell. Data shown represent the mean ± SEM of the 20 images and were normalized to the total fluorescence intensity (plasma membrane + intracellular compartment) set to 100% in each condition.