Table 3.
Apparent rates of dipeptide formation
Preassembled complex | Start codon | kapp, s−1 | |||
WT | ΔH69 | Δloop | Δloop+4 | ||
30SIC | AUG | 0.34 ± 0.04 | 0.018 ± 0.003 | 0.087 ± 0.011 | 0.085 ± 0.02 |
AUC | 0.017 ± 0.005 | 0.0025 ± 0.0006 | 0.0077 ± 0.0033 | 0.0068 ± 0.0013 | |
30SIC-IF3 | AUG | 0.82 ± 0.13 | 0.20 ± 0.02 | 0.14 ± 0.02 | 0.16 ± 0.02 |
AUC | 0.62 ± 0.26 | 0.16 ± 0.03 | 0.12 ± 0.01 | 0.10 ± 0.01 | |
70SIC | AUG | 2.0 ± 0.3 | 0.032 ± 0.006 | 0.14 ± 0.01 | 0.17 ± 0.05 |
70SIC-IF1 | AUG | 1.7 ± 0.3 | 0.038 ± 0.004 | 0.14 ± 0.01 | 0.16 ± 0.02 |
70SIC-IF3 | AUG | 1.9 ± 0.1 | 0.44 ± 0.14 | 0.19 ± 0.05 | 0.18 ± 0.03 |
70S | AUG | 2.9 ± 0.6 | 1.8 ± 0.1 | 1.4 ± 0.3 | 1.3 ± 0.3 |