Table S1.
Data collection, Ni-SAD phasing, and refinement statistics
| EvdO1 | EvdO1 Ni-SAD | EvdO2 | EvdO2-AKG | AviO1 | AviO1 Ni-SAD | |
| Data collection | ||||||
| PDB ID code | 4XBZ | 4XAB | 4XAC | 4XAA | ||
| Space group | P21 | P21 | P212121 | P212121 | P21212 | P21212 |
| Cell dimensions | ||||||
| a, b, c (Å) | 91.6, 109.4, 146.1 | 92.3, 109.0, 146.2 | 42.6, 65.8, 84.5 | 42.5, 65.4, 84.6 | 72.1, 115.1, 33.9 | 72.4, 115.4, 33.9 |
| α, β, γ (°) | 90, 108.3, 90 | 90, 108.3, 90 | 90, 90, 90 | 90, 90, 90 | 90, 90, 90 | 90, 90, 90 |
| Wavelength, Å | 0.979 | 1.485 | 1.078 | 1.127 | 1.485 | 1.485 |
| APS Beamline | 21-ID-F | 21-ID-D | 21-ID-D | 21-ID-D | 21-ID-D | 21-ID-D |
| Resolution, Å | 2.3 | 2.9 | 1.75 | 1.87 | 2.3 | 2.5 |
| Rsym, % | 12.1 (41.8) | 8.6 (44.3) | 6.8 (29.6) | 7.4 (32.4) | 7.0 (44.0) | 11.1 (92.6) |
| I/σI | 15.2 (2.3) | 25.9 (6.2) | 18.1 (4.2) | 22.3 (5.8) | 26.7 (2.6) | 34.4 (2.5) |
| Completeness, % | 99.5 (99.1) | 98.1 (97.0) | 99.9 (99.6) | 97.2 (98.7) | 86.7 (61.1) | 98.6 (97.3) |
| Redundancy | 3.8 (3.1) | 7.7 (7.8) | 3.6 (3.6) | 5.7 (5.7) | 4.0 (2.8) | 8.5 (5.9) |
| Refinement | ||||||
| Resolution, Å | 2.3 | NA | 1.75 | 1.87 | 2.3 | NA |
| No. of reflections | 120,893 | NA | 24,667 | 19,679 | 11,436 | |
| Rwork/Rfree | 18.3/23.7 | 17.5/20.2 | 16.8/20.8 | 18.8/22.1 | ||
| No. of atoms | ||||||
| Protein | 19105 | 2006 | 2014 | 1700 | ||
| Ligand/ion | 44 | 11 | 16 | 1 | ||
| Water | 1333 | 182 | 144 | 62 | ||
| B-factors | ||||||
| Protein | 35.1 | 28.3 | 34.3 | 46.9 | ||
| Ligand/ion | 44.3 | 28.2 | 27.8 | 58.2 | ||
| Water | 38.5 | 36.2 | 41.3 | 46.5 | ||
| Ramachandran | ||||||
| Favored, % | 96.0 | 98.0 | 98.0 | 97.3 | ||
| Allowed, % | 3.8 | 2.0 | 2.0 | 2.7 | ||
| Outliers, % | 0.2 | 0.0 | 0.0 | 0.0 | ||
| rms deviations | ||||||
| Bond lengths, Å | 0.008 | 0.006 | 0.007 | 0.008 | ||
| Bond angles, ° | 1.07 | 1.05 | 1.11 | 1.10 |
All datasets were collected from a single crystal. Values in parentheses are for data in the highest-resolution shell. The EvdO1 Ni-SAD dataset was used only for phasing. A subset of frames from the AviO1 Ni-SAD dataset was used for refinement. NA, not applicable.