Fig. 4. DEPDC1 is not associated with centrosome. (A) Overexpression of DEPDC1 in HeLa cells. HeLa cells were transiently transfected with an empty vector or the expression plasmid for Flag-DEPDC1. Twenty four hours after transfection, whole cell lysates were prepared from unsynchronized or synchronized HeLa cells and subjected to immunoblotting. (B) HeLa cells were transiently transfected with the expression plasmid for Flag-DEPDC1. Twenty four hours after transfection, cells were fixed and stained with anti-γ-tubulin antibody (green) and anti-Flag antibody (red). DAPI was used to stain cell nuclei (blue). Scale bar, 20 μm. (C) Knockdown of DEPDC1 results in the upregulation of A20. HeLa cells were transiently transfected with the negative control siRNA (NC siRNA) or with siRNA against DEPDC1. Forty-eight hours after transfection, total RNAs were prepared and subjected to quantitative RT-PCR.
