Figure 1.

PpCTR1L is related to CTR1 homologs and CTR1-like proteins. AtCTR1, AtEDR1, and their related sequences form a monophyletic group, which is further subdivided into the EDR1 and CTR1 gene families. Within the CTR1 gene family, angiosperm sequences form two distinctive subfamilies labeled as CTR1 and CRG, while early land-plant CTR1-like sequences are placed outside of these two subfamilies. The tree was constructed using the maximum likelihood method on aligned amino acid sequences listed in Supplemental Table S1. Bootstrap support values are indicated at internal nodes. The scale bar indicates the number of changes per site. Sequences from P. patens are shown in red, those from Marchantia polymorpha in blue, and those from S. moellendorffii in green. The asterisk indicates the node at which the EDR1 and CTR1 protein families join together.