Figure 2.

PpCTR1L can bind to ethylene receptors. A quantitative yeast two-hybrid assay for interactions between AtCTR1, PpCTR1L, and ethylene receptors from moss and Arabidopsis is shown. The transmembrane domains of the ethylene receptors and the kinase domains of CTR1-related proteins were excluded from the constructs to avoid these domains interfering with the assay. Results are expressed as means ± sd (n = 3), and the experiment was conducted twice. AD, Activation domain; BD, DNA binding domain.