Figure 10.

Overexpression of Gβγ dimers sequesters XLG3 at the plasma membrane. XLG3::mVenus driven by the UBIQUITIN10 promoter is retained at the plasma membrane when coexpressed with 35S-driven AGB1-AGG1 (A) or AGB1-AGG2 (B) dimer. (Compare with localization of the XLG3::mVenus fusion protein in the absence of additional Gβγ in Figure 7, E and F.) Note the lack of nuclear signal in two nuclei (white arrows) visible in the bright-field channel. The plasma membrane retention was consistent over time; these images were acquired 6 d post infiltration of N. benthamiana leaves at a final OD₆₀₀ of 0.0075 for XLG3 and 0.025 for Gβγ dimers. Yellow indicates mVenus, blue indicates mTq2, and red indicates chlorophyll autofluorescence. Bars = 50 μm.