Figure 14.

agb1, agg1 agg2, and the xlg triple mutants display increased stomatal density. Propidium iodide-stained cotyledons of 9-d-old seedlings were imaged using a confocal microscope, and images were used to quantify stomatal density for mutants of the Gγ subunits (A) and xlg mutants (B). The assay was conducted for all genotypes simultaneously, and the Col-0, agb1-2, and gpa1-3 controls represent the same data in A and B. Significant differences from Col-0 (Student’s t test) are indicated: *, P < 0.05 to 0.01; and **, P < 0.01 to 0.001. All values are means ± se. Representative images of propidium iodide-stained cotyledons are shown for Col-0 (C), gpa1-4 (D), agb1-2 (E), and the xlg triple mutant (F). White indicates propidium iodide stain. Bars = 50 µm.