Figure 4.

The XLG3 Gα-like region aligns closely to the crystal structure of GPA1. Superimposition of computationally derived XLG3 structural features on the empirically derived GPA1 crystal structure demonstrates their shared three-dimensional characteristics. Blue indicates the XLG3 Gα-like region, orange indicates the GPA1 Gα-like region, and N- indicates the N terminus of the Gα regions. Thr-132 marks the NVen210 insertion site in the αB-αC loop of GPA1 used for BiFC (Gookin and Assmann, 2014): each of the XLGs was modified at the analogous residue.