Figure 7.

Subcellular localization of Arabidopsis XLG proteins in N. benthamiana leaves. A to D, UBIQUITIN10 promoter-driven XLG:mVenus fusions show differing localization patterns. A and B, XLG1 is predominantly extranuclear with nearly undetectable (A) to very weak (B) nuclear signal. C and D, XLG2 varies between strong nuclear signal (C) and signal evenly divided between the nucleus and cytoplasm (D). E and F, XLG3 consistently localizes to the nucleus and the cytoplasm. Agroinfiltrations of N. benthamiana leaves were performed at an OD₆₀₀ of 0.0075 to 0.01 and imaged at 60 to 68 h, except for F, which was imaged at 6 d post infiltration. Yellow indicates mVenus, blue indicates mTq2, and red indicates chlorophyll autofluorescence. Bars = 50 μm.