Figure 9.

XLG3 has a functional NES. A, The N-terminal domain of XLG3 has an unconventional NES (triangle) located just upstream of the Gα-like region. The XLG3 NES was mutated by substituting hydrophobic residues at the N terminus (XLG3m1) or the C terminus (XLG3m2) of the domain with Gly residues. Both mutants showed nearly exclusive nuclear localization. B and C, XLG3m1 nuclear localization in two focal planes of the same mesophyll cells. D, XLG3m2 nuclear localization in epidermal pavement cells. Agroinfiltrations of N. benthamiana leaves were performed at an OD₆₀₀ of 0.0075 to 0.01 and imaged 50 to 55 h later. Yellow indicates mVenus, blue indicates mTq2, and red indicates chlorophyll autofluorescence. Bars = 50 μm.