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. 2015 May 20;169(1):166–179. doi: 10.1104/pp.15.00335

Figure 4.

Figure 4.

ERF6 and ERF11 antagonistically regulate common target genes. A, Expression levels of ERF6, ERF11, and the ERF6 target genes in dexamethasone-inducible overexpression lines of ERF6 (ERF6-GR), ERF11 (ERF11-GR), and both in ERF6-GR × ERF11-GR double homozygous plants. Expression values are the average of at least three biological repeats. Values are relative to the expression level in wild-type plants subjected to the same treatment. B, Protoplast activation assay with pSTZ:fLUC, pWRKY33:fLUC, and pMYB51:fLUC for binding and effect of ERF6, ERF11, and a combination of both in a 1:1 ratio. C, Protoplast activation assay on the promoter of MYB51 for the native ERF11 protein, a truncated ERF11 in which the repressive EAR domain has been removed, and a combination in a 1:1 ratio of ERF6 and the truncated ERF11. D, Titration protoplast activation assay on the promoter of MYB51 with multiple concentrations of ERF6 and ERF11. For B to D, values are signal intensities normalized to the cotransformed normalization plasmid rLUC and relative to the negative control (35S:GUS + pTARGET:fLUC, horizontal line). Values represent the average of at least two biological repeats with their se. FC, Fold change.