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. 2015 May 20;169(1):148–165. doi: 10.1104/pp.15.00353

Figure 5.

Figure 5.

Knockdown of both MHZ6/OsEIL1 and OsEIL2 expression by RNAi (OsEIL1-2-RNAi) confers ethylene insensitivity in roots and coleoptiles. A, Expression levels of MHZ6/OsEIL1 and OsEIL2 in OsEIL1-2-RNAi transgenic lines compared with the wild type (WT) by semiquantitative PCR. OsACTIN1 was amplified as a loading control. B, Ethylene-response phenotypes of the wild type, osein2/mhz7-1, mhz6-1, OsEIL2-RNAi-19, and three OsEIL1-2-RNAi lines. The etiolated seedlings were grown in air or 10 µL L−1 ethylene for 3 d. Bar = 10 mm. C, Length of roots in the wild type, osein2/mhz7-1, mhz6-1, OsEIL2-RNAi-19, and three OsEIL1-2-RNAi lines in response to ethylene. Values are means ± sd (n ≥ 30). D, Length of coleoptiles in the wild type, osein2/mhz7-1, mhz6-1, OsEIL2-RNAi-19, and three OsEIL1-2-RNAi lines in response to ethylene. Values are means ± sd (n ≥ 30).