Figure 6.

zmbri1-RNAi plants resemble Rs1-O mutants. A, zmbri1-RNAi. B, Rs1-O. C, BR-related gene expression in the auricle region of developing Rs1-O mutant leaves. The dwf4 and zmbri1b genes show altered expression in the mutant. D, KNOX rs1 gene expression was not altered in the auricle region of developing zmbri1-RNAi leaves. qRT-PCR was performed on RNA isolated from auricle regions of wild-type (WT) versus mutant or RNAi lines. Two millimeters of leaf tissue was dissected from the auricle region of plastochron 7 to 10 leaves from 10-leaf stage plants. *, P < 0.05; and **, P < 0.01 by Student’s t test.