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. 2015 Jul 23;169(1):793–802. doi: 10.1104/pp.15.00547

Figure 2.

Figure 2.

AvrE is localized at the PM and PM-associated vesicle-like structures in the plant cell. A, Subcellular fractionation of His:AvrE expressed in transgenic Arabidopsis plants. C, Crude plant extract; T, total plant protein; S, soluble protein fraction; M, total membrane protein fraction. The red arrow indicates the His:AvrE band. B and C, Confocal images of His:YFP:AvrE expressed in leaf cells of transgenic Arabidopsis plants. The yellow arrow indicates the PM. C is a z-stacked image. D, Confocal image (z-stack) of His:YFP:AvrE expressed in leaf cells of transgenic Arabidopsis plants after plasmolysis using 0.5 m NaCl. The red arrow indicates PM Hechtian strands, and the yellow arrows indicate the PM. E, Confocal image (z-stack) of AvrE:VFP expressed in N. benthamiana leaf cells. F and G, Confocal images of His:YFP:AvrE-ee (F) and His:YFP:AvrE-kk (G) expressed in leaf cells of transgenic Arabidopsis plants. Bars = 10 µm.