NHL13 is required for Arabidopsis immunity. A, The nhl13-1 mutant plant showed enhanced susceptibility to the avrE hopM1 mutant. Five-week-old Col-0 and nhl13-1 plants were dip inoculated with Pst DC3000, the avrE hopM1 mutant, or the hrcC mutant at 1 × 108
cfu mL−1. The bacterial population was counted 4 d after inoculation. Asterisks indicate significant differences in bacterial multiplication when compared with that in Col-0 plants: **, P < 0.01; and *, P < 0.05. B, Results of qRT-PCR to measure the levels of the NHL13 transcript in response to inoculation with Pst DC3000, the avrE mutant, the hopM1 mutant, the avrE hopM1 mutant, or water. The level of NHL13 was normalized to that of PROTEIN PHOSPHATASE 2A SUBUNIT A3 (PP2AA3; At1G13320).