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. 2015 Jul 14;169(1):760–779. doi: 10.1104/pp.15.00575

Figure 5.

Figure 5.

rcn1-6/pp2ac and pp2ac double mutants exhibit altered ABA responses during seed germination and seedling growth. A and B, Time-dependent seed germination of Col-0 and indicated pp2a double mutants in the presence of 0 (A) or 0.8 μm ABA (B). C and D, Seed germination on day 4 after stratification (C) and cotyledon expansion on day 6 after stratification (D) in the presence of 0 (blue bars) or 0.8 μm ABA (red bars). A to D, Means ± sem (n = 4) with 49 seeds per n normalized to the seed count. E, Four-day-old seedlings were transferred to one-half-strength MS agar plates supplemented with 0 (upper) or 5 μm ABA (lower) and grown for an additional 5 d. F, Root growth of seedlings shown in E in the presence of 0 (blue bars) and 5 μm ABA (red bars; means ± sem [n = 5] with seven seedlings per n) normalized to the 0 μm ABA control conditions. G, Stomatal apertures 2 h after incubation in 0 (blue bars) or 5 μm ABA (red bars; means ± sem [n = 4] with ≥19 stomata per n) normalized to the 0 μm ABA control conditions. Statistical values for differences between the Col-0 wild type and the pp2a double-mutant lines were calculated using two-way ANOVA. *, P < 0.05; **, P < 0.01; ***, P < 0.001.