Figure 8.

Analyses of PP2A-type protein phosphatase and SnRK2-type protein kinase cross regulation. A, PP2A-type protein phosphatase activity of 2-week-old GFP control seedlings and PP2AB′beta-YFP using MBP as a substrate. Activities were measured after GFP complex isolations 1 h after 0 μm ABA (blue bars) or 50 μm ABA (red bars) treatments. Data represent means ± sd of n = 3 experiments. B, In-gel kinase assay of 1-week-old Col-0 wild type and indicated pp2a double mutants in response to 1 h of 0 μm ABA (−) or 10 μm ABA (+) treatment. Coomassie Brilliant Blue (CBB) staining indicates the protein loading.