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. 2015 Jul 21;169(1):780–792. doi: 10.1104/pp.15.00623

Figure 2.

Figure 2.

Isolation and complementation of the cipk21 T-DNA insertional mutant. A, Scheme representing the Arabidopsis CIPK21 gene. Exons (black boxes) and introns (lines) are indicated. The position of the T-DNA insertion is indicated by a triangle (not represented to scale). B, Genomic DNA fragment used for complementation. A 4.5-kb fragment including the CIPK21 coding region and 1.6 kb of the 5′ flanking DNA upstream of the start codon and 3′ untranslated region was amplified by PCR and cloned into the pCAMBIA 1300 for plant transformation. C, RT-PCR analysis of CIPK21 gene expression from wild-type (WT), cipk21, and cipk21/CIPK21 plants. Expression of ACTIN2 was analyzed as a loading control.