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Colocalization of CIPK21 with CBL2 and CBL3 proteins in the epidermal peel cells of N. benthamiana. CIPK21:GFP coexpressed with either CBL2:mCherry (top row, red) or CBL3:OFP (bottom row, red) display the formation of CBL2/CIPK21 and CBL3/CIPK21 complexes preferentially at the vacuolar membrane, as indicated by the yellow color in the overlay image (right column). Bar = 40 µm.
