Figure 1.

The ETR1 receiver domain is not required for response to silver ions. A, Domain structure of full-length and truncated ETR1 receptor lacking the receiver domain used in this study. B, Growth kinetic profiles in the presence of 100 µm AgNO₃ are shown for etr1-6;etr2-3;ein4-4 triple mutants transformed with cDNA encoding for either a full-length ETR1 (cETR1) or a truncated transgene lacking the receiver domain (cetr1-ΔR). For comparison, responses of wild-type and triple mutant seedlings are shown. Seedlings were allowed to grow for 1 h in air, followed by the addition of 1 µL L^–1 ethylene (down arrow). Growth rate was normalized to the growth rate in the air pretreatment and represents the average ± sem. Lines are a moving average calculated with a period of 2 using Microsoft Excel.