Figure 7.

ABA changes the transcript abundance of RAB18 and CRA1. The levels of transcript for CRA1 and RAB18 were measured using qRT-PCR. For this, seeds were germinated for 2 d in the presence or absence of 1 µm ABA and mRNA extracted from the wild type and etr1-6;etr2-3;ein4-4 triple mutants as well as triple mutants transformed with the indicated mutant transgene. Data were normalized to the levels of At3g12210 in each seed line to determine the relative transcript level for each gene. These were then normalized to levels of the transcript in untreated wild-type seeds. The average ± sem for two biological replicates with three technical replicates each is shown. The letter a indicates that ABA caused a statistically significant increase in transcript levels (P < 0.05), and the letter b indicates a statistical difference from wild-type seeds in the same condition (P < 0.05). All P values were calculated by Student’s t test.