Table I. Summary of rescue of traits by ETR1 transgenes.
etr1-6;etr2-3;ein4-4 Transformed with: | Phenotype Rescueda |
|||
---|---|---|---|---|
Growth in Airb | Growth Recovery after Ethylene Removalc | Ethylene-Stimulated Nutationsb | Germination Rate during Salt Stressd | |
gETR1 | +++ | +++ | +++ | +++ |
E617A | +++ | +++ | + | +++ |
N618A | +++ | ++++ | + | +++ |
D659A | +++e | +e | +++f | + |
C661A | +++ | ++ | + | + |
V665A | ++ | ++ | + | +++++ |
E666A | ++ | +++ | + | +++++ |
N667A | +++ | +++ | + | ++ |
Q681A | ++ | ++++ | + | ++++ |
R682A | ++ | ++ | + | +++ |
Q684A | ++ | ++++ | – | ++ |
E730A | ++ | ++++ | – | ++ |
L734A | ++ | +++ | – | ++ |
Rescue for each mutant transgene was scored relative to the rescue obtained with the gETR1 transgene.
Results of growth in air and nutations (Fig. 3) were scored as follows: more than 70% rescue, +++; 50% to 70% rescue, ++; 30% to 50% rescue, +; and no rescue, –.
Results for growth recovery (Fig. 3) were scored as follows: recovery time was faster, ++++; recovery time was comparable, +++; recovery time was slightly slower, ++; and recovery time was very slow and only slightly faster than the etr1-6;etr2-3;ein4-4 mutants, +.
Results for the time for 50% germination on salt (Supplemental Fig. S1) were scored as follows: more than 65% slower, +++++; 20% to 65% slower, ++++; comparable, +++; 15% to 35% faster, ++; and more than 35% faster, +.