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. 2015 Jul 8;169(1):814–825. doi: 10.1104/pp.15.00782

Figure 2.

Figure 2.

Effect of YHB on bioluminescence rhythms in constant darkness. A and C, Bioluminescence of Col-0 (solid) and YHB (dashed) seedlings containing a CCR2::LUC reporter grown on MS medium with (A) or without (C) exogenous Suc; n > 9. B and D, Bioluminescence of Ler (solid) and YHB (Ler; dashed) seedlings containing a CCA1::LUC2 reporter grown on MS medium with (B) or without (D) exogenous Suc; n > 10. YHB (Ler) is presented on a secondary axis in D for clarity. E and F, Abundance of CCR2 transcripts as measured by quantitative reverse transcription (qRT)-PCR after transfer of seedlings to constant darkness. Plants were grown with (F) or without (E) exogenous Suc as described in A and C. G and H, Abundance of CCA1 transcripts as measured by qRT-PCR after transfer of seedlings to constant darkness. Plants were grown with (H) or without (G) exogenous Suc as described in A and C. Plants were entrained under 60 μmol m−2 s−1 white light in 12:12 L/D cycles for 6 d and then transferred to constant darkness at ZT12 on day 6. Gray bars indicate subjective day, whereas black bars indicate subjective night. CCR2 and CCA1 mRNA levels were normalized to PP2a. sem is shown.