Table II. Linkage analysis of total mucilage extracts.

Total mucilage was extracted by vigorous mixing in water. Values represent the relative composition (mol %) of each linkage ± sd of three biological replicates. Asterisks indicate significant differences (Student’s t test, P < 0.05) from the wild type.

Linkage	Wild Type	muci10-1
Rha
t-Rha	0.49 ± 0.19	0.31 ± 0.19
2-Rha	36.81 ± 0.38	36.69 ± 3.30
2,3-Rha	0.66 ± 0.11	0.86 ± 0.42
2,4-Rha	0.85 ± 0.24	1.31 ± 1.15
Ara
t-Ara	0.07 ± 0.03	0.06 ± 0.01
3-Ara	0.41 ± 0.04	0.74 ± 0.29
5-Ara	0.19 ± 0.04	0.23 ± 0.03
Gal
t-Gal	0.71 ± 0.09	0.13 ± 0.05*
2-Gal	0.11 ± 0.01	0.03 ± 0.02*
4-Gal	0.11 ± 0.00	0.11 ± 0.04
6-Gal	0.21 ± 0.11	0.17 ± 0.07
2,4-Gal	0.08 ± 0.01	0.08 ± 0.04
4,6-Gal	0.05 ± 0.03	0.03 ± 0.02
3,6-Gal	0.29 ± 0.05	0.55 ± 0.39
Glc
t-Glc	0.01 ± 0.01	0.00 ± 0.00
4-Glc	1.07 ± 0.03	0.73 ± 0.10*
3,4-Glc	0.01 ± 0.01	0.02 ± 0.00
4,6-Glc	0.07 ± 0.01	0.06 ± 0.02
Xyl
t-Xyl	0.43 ± 0.05	0.42 ± 0.06
4-Xyl	1.61 ± 0.11	1.60 ± 0.04
2,4-Xyl	1.33 ± 0.04	1.45 ± 0.12
Man
4-Man	0.25 ± 0.01	0.34 ± 0.03*
4,6-Man	0.53 ± 0.12	0.14 ± 0.09*
GalUA
t-GalA	0.80 ± 0.33	0.56 ± 0.23
4-GalA	51.39 ± 0.66	50.78 ± 0.57
2,4-GalA	0.56 ± 0.11	0.73 ± 0.36
4,6-GalA	1.24 ± 0.16	1.80 ± 1.21