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Quantitative real-time PCR analysis of the expression of four genes encoding 2S precursors in developing seeds of wild-type (Ler) and ttg1-1 plants. Results were normalized against the expression levels of EF1aA4 as an internal control. Asterisks indicate significant differences in gene expression levels in ttg1-1 compared with those in wild-type plants (two-tailed paired Student’s t test, P < 0.05). Error bars denote sd.
