Figure 10. Association kinetics of GreB with transcription elongation complexes.

(A) Cumulative fractions of surface-tethered elongation complex targets (blue; N = 287) and control sites (black; N_c = 382) at which GreB (0.5 nM) co-localized at least once prior to the indicated time. Fitting (see text) was used to calculate model curves based on Eq. 5 (red; k_a = (1.49 ± 0.13) × 10⁻² s⁻¹ and A_f = 0.83 ± 0.02) and an exponential probability density function (cyan; k_ns = (1.75 ± 0.19) × 10⁻⁴ s⁻¹). (B) Estimated uncertainty in k_a. The plot is a histogram of k_a values derived from fitting 5,000 bootstrap samples of the data in (A). The standard deviation of these values (0.13 × 10⁻² s⁻¹) is the estimated standard error of k_a reported in (A).