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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: J Virol Methods. 2015 Aug 10;224:20–29. doi: 10.1016/j.jviromet.2015.07.023

Table 1.

Primer sequences used for RT-PCR and cDNA cloning.

Target RNA Forward primer Reverse primer Amplicon (bp)
HIV-1 gRNA 5′-TGTGTGCCCGTCTGTTGTGT-3′ 5′-GAGTCCTGCGTCGAGAGAGC-3′ 143
HIV-1 Tat 1-86 5′-GGAAGCATCCAGGAAGTCAGCCTA-3' 5′-GGGTTGGGAGGTGGGTTGCTT-3' 170
7SL 5′-ATCGGGTGTCCGCACTAAG-3′ 5′-CACCCCTCCTTAGGCAACCT-3′ 81
Y4 5′-AAGCCAGTCAAATTTAGCAGTGGG-3′ 5′-AAGCCAGTCAAATTTAGCAGTGGG-3′ 93
Y1 5′-GGCTGGTCCGAAGGTAGTGA-3′ 5′-AAAAGACTAGTCAAGTGCAGT-3′ 113
GAPDH 5′-CATGGCACCGTCAAGGCTGAGAA-3′ 5′-CAGTGGACTCCACGACGTACTCA-3' 130
β-Actin 5′-CTGGCACCACACCTTCTACAATGA-3′ 5′-GCTGGGGTGTTGAAGGTCTCAAA-3′ 137
EEF2 5′-ATCCGCGCCATCATGGACAAGAA-3' 5′-TCGTCCTTCCGGGTATCAGTGAA-3' 164
EndoG 5′-AGGCCATGGACGACACGTTCTAC-3′ 5'-GAAGAGTGGCCCTGTGCAGACATA-3' 146
CPSF100 5′-GCCATCAGCTCATAAGACGAAGCAT-3′ 5′-AGTAGC TTGAAGCTCTGGCACTAAG-3' 187
aHIV-1 Tat1-86 (full-length) 5′-ATGGAGCCAGTAGATCCTAGA-3′ 5′-CTATTCCTTCGGGCCTGTC-3′ 261
amEndoG 5′-CACCATGGACGACACCTTCTACC-3′ 5′-CTTGCTGCCAGCAGTGATAGC-3′ 432
(C-terminal)
a

Primers for cDNA cloning.