Fig. 4.

miR-26a increases the expression of Beclin-1 by the activation of MAPKs. a Expression levels of Beclin-1 and LC3 were measured by qRT-PCR in cells transfected with miR-26a for 24 h. b Cells were transfected with miR-26a or scramble miRNA (miR-NC) for 48 h. Lysates of treated cells were subjected to immunoblotting. Beclin-1 was quantified by ImageJ densitometric analysis and normalized using GAPDH. Results shown are the mean ± SD of at least three independent experiments. *P < 0.05; **P < 0.01 compared with cells transfected with scramble miRNA