Fig. 5.

miR-26a targets several genes involved in the induction of autophagy. a Diagram of the 3′-UTRs of DUSP4 and DUSP5 in different species. b Luciferase reporter assay of psicheck2.2 with 3′-UTR fragments of DUSP4 and DUSP5. c, d mRNA and protein levels of DUSP4 and DUSP5 in Huh7 cells transfected with miR-26a. e Luciferase reporter assay of psicheck2.2 with 3′-UTR fragments of potential targets. f Real-time PCR analysis of miR-26a target genes after transfection with the miR-26a precursors. Results shown are the mean ± SD. *P < 0.05; **P < 0.01 compared with cells transfected with scramble miRNA