Fig. 4.

Immunohistochemical localization of DDC in brain sections of chronically infected mice with horseradish peroxidase labeling. Brown staining indicates the presence of DDC. Hematoxylin was used to counterstain the sections and highlight cell nuclei blue. T. gondii tissue cysts (labeled with an asterisk) containing multiple individual parasites are large and circular. (A, B) show negative AADC staining (no primary antibody, secondary only was applied) in the hippocampus (hc) at 20× and 40× magnification, respectively. (D, E) show positive DDC staining in the hippocampus at 20× and 40× magnification, respectively. (C) shows negative DDC tissue staining in the cerebellum (crb), while (F) displays a positive tissue control for DDC staining at the border of the striatum (st) and cortex (cx). Regions of hippocampus (hc), cerebellum (crb), striatum (st), and cortex (cx) are labeled. Scale bars = 50 μm.