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. 2015 Sep 21;212(10):1513–1528. doi: 10.1084/jem.20141831

Figure 1.

Figure 1.

IEC-intrinsic IKKα, but not IKKβ, expression is critical for immunity to C. rodentium infection. (a) IKKβ and IKKα expression in IECs from naive IKKβF/F, IKKαF/F, IKKβΔIEC, and IKKαΔIEC mice as detected by Western blot. (b–j) Littermate control IKKβF/F and IKKαF/F mice and mutant IKKβΔIEC and IKKαΔIEC mice were infected with C. rodentium. (b–d) C. rodentium CFU in the feces on day 5 p.i. (b), spleen on day 11 p.i. (c), and liver on day 11 p.i. (d). (e and f) Percentage of initial body weight (e) and percent survival (f) at the indicated time points p.i. (g) H&E staining of colon tissue sections from naive mice. (h) H&E staining of colon tissue sections from C. rodentium–infected mice at day 11 p.i., including high-magnification insets (bottom). (i) Pathological score of colon histology. (j) H&E staining of liver tissue sections of day 11 infected mice. Arrows indicate neutrophil-rich inflammatory foci. All bars, 50 µm. Data for a are representative of two independent experiments using pooled IECs from three mice. Data for b–j are representative of three to four independent experiments (IKKβF/F, total n = 13; IKKαF/F, n = 18; IKKβΔIEC, n = 12; and IKKαΔIEC mice, n = 16). Data for f are pooled from three independent experiments. Data are shown as mean ± SEM. † indicates infection-induced mortality. *, P < 0.05 compared with IKKαF/F.