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. 2015 Sep 21;212(10):1513–1528. doi: 10.1084/jem.20141831

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© 2015 Giacomin et al.

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Figure 4. — C. rodentium infection–induced ILC-dependent IL-22 responses are diminished in IKKα^ΔIEC mice. IKKα^F/F mice were infected (INF) with C. rodentium and sacrificed at day 4 p.i. (a) Representative plots displaying frequencies of IL-22⁺ cells in the mLNs. (b) Expression of T cell (CD3 and CD5) and NK cell (NK1.1) markers among IL-22⁺ cells. (c) Expression of ILC surface markers in gated CD3⁻CD5⁻NK1.1⁻IL-22⁺ cells (black, open histograms) compared with CD19⁺ B cells (gray, closed histograms). (d) Representative plots displaying frequencies of CD3⁻CD5⁻CD19⁻CD11c⁻NK1.1⁻, RORγt⁺CD25⁺ ILCs in the mLNs of naive and C. rodentium–infected littermate control IKKα^F/F and IKKα^ΔIEC mice. (e) Total mLN ILC3s. (f) Frequencies of IL-22–expressing RORγt⁺ ILCs in the mLN after ex vivo PMA and ionomycin stimulation. (g) Frequencies of IL-22–expressing RORγt⁺ ILCs in the mLNs after stimulation with rIL-23, PMA, and ionomycin. (h) Frequencies of CD3⁺CD4⁺ T cells in the mLNs expressing IL-22 after ex vivo PMA and ionomycin stimulation. Data for a–f and h are representative of five experiments (IKKα^F/F, total n = 21; IKKα^ΔIEC, n = 17 + 1 naive mouse of each genotype per experiment), and data for g are representative of three experiments (IKKα^F/F, total n = 13; IKKα^ΔIEC, n = 11 + 1 naive mouse of each genotype per experiment). Data are expressed as mean ± SEM. *, P < 0.05 compared with IKKα^F/F INF.