Figure 9.
IEC-intrinsic IKKα expression regulates inflammation and pathology during chemically induced colitis. Littermate IKKαF/F and IKKαΔIEC mice were treated with either 3% DSS in the drinking water or maintained on normal drinking water (naive, N). (a) Percentage of original body weight. (b) Gross disease score. (c) Colon length at day 6 after DSS. (d) H&E staining of colon tissue sections of naive and DSS-treated mice at day 6 after DSS. Bar, 50 µm. (e) IFNγ, IL-17A, and IL-22 protein expression in overnight colonic organ culture supernatants at day 4 after DSS. Data for a–d are representative of three independent experiments (IKKαF/F, n = 17; IKKαΔIEC, n = 14 + 2 naive of each genotype per experiment). Data for e are representative of two independent experiments (IKKαF/F, n = 8; IKKαΔIEC, n = 8 + 2 naive of each genotype per experiment). Data are expressed as mean ± SEM. *, P < 0.05 compared with IKKαF/F + DSS.