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. 2015 Sep 21;212(10):1487–1495. doi: 10.1084/jem.20150303

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© 2015 Tang et al.

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Figure 3. — CystLTR1 and LTC4S are up-regulated by IL-15 and NKG2D in a dose-dependent manner. (A) Fresh IE-CTLs were cultured overnight and stimulated with the indicated concentration of IL-15 for 3 h. Cell lysates were immunoblotted with antibodies against CystLTR1 or LTC4S. Blots are representative of four independent experiments. Bottom panels show mean fold change ± standard deviation of four independent experiments. (B) Fresh IE-CTLs were cultured as in A but were stimulated with an antibody against NKG2D for 3 h in lieu of IL-15. Lysates were isolated and immunoblotted as in A. β-Actin is shown as a loading control. Blots are representative of four independent experiments, and the bottom panels present means ± standard deviation of four independent experiments.