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. 2015 Sep 21;212(10):1497–1512. doi: 10.1084/jem.20150496

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© 2015 Calderon et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 9. — Pancreatic macrophage profiles after genotoxic insult. (A and B) Stroma from WT CD45.2 (A) and CD45.2 mice receiving CD45.1 BMT (CD45.1 BMT; B) after genotoxic insult and evaluated at 9 wk after transplant. (top) Stroma cells (gated on singlets CD45⁺) showing F4/80 and CD11b profile. (middle) Histograms of F4/80⁺ CD11b⁺ gated cells (from top panels) evaluated for the presence of CD45.1⁺ (donor) and CD45.1⁻ (host) macrophages. (bottom) Histograms of F4/80⁺ CD11b⁺ gated cells from CD45.2 WT and CD45.1 BMT showing CD206 and CD301 expression. Data are a representative of two independent experiments. (C) Sorted islet macrophages (CD45⁺ F4/80⁺ CD11b⁺) and stromal macrophages (CD45⁺ F4/80⁺ CD11b⁺ CD206⁺ and CD45⁺ F4/80⁺ CD11b⁺ CD206⁻) from CD45.2 WT and CD45.1 BMT mice at 9 wk after transplant. CD45-negative (CD45neg) islet cells were used as controls for comparison. M1-like (Il1b and Tnfa), M2-like (Il10, Arg1, Mrc1, Mgl1, Mgl2, Ym1, and Fizz1), and control (Ins1, Ptprc, and Emr1) transcripts were assessed. Transcript expression is pooled from two independent experiments performed in duplicate. Bars represent the mean ± SEM. P-values were calculated using the unpaired Student’s t test: ***, P < 0.001; **, P < 0.01; *, P < 0.05; NS, P ≥ 0.05. Sample size included two samples per experimental group.