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. 2015 Sep 21;212(10):1679–1692. doi: 10.1084/jem.20150489

Figure 1.

Figure 1.

miR-132 is induced in B cells in response to BCR signaling. (A) Relative miR-132 expression in primary splenic B cells stimulated with anti-IgM (aIgM; 5 µg/ml), LPS (20 µg/ml), CD40L (2 µg/ml), IL-4 (10 ng/ml), or CpG (0.5 µM) for the indicated durations. miR-132 expression was detected using TaqMan RT-qPCR (n = 3). (B) Relative expression of miR-212 in splenic B cells stimulated with LPS (20 µg/ml) or anti-IgM (5 µg/ml) for the indicated times (n = 3). (C) Relative expression of miR-132 in splenic B cells stimulated for 8 h with a combination of PMA (15 ng/ml), ionomycin (0.5 µg/ml), and anti-IgM (5 µg/ml), as indicated (n = 3). (D) Relative expression of miR-132 in splenic B cells stimulated for 8 h with varying concentrations of anti-IgM as indicated (n = 3). Data represent two independent experiments and are represented as mean ± SEM. ***, P < 0.001, Student’s t test.