Skip to main content
. 2015 Sep 21;212(10):1679–1692. doi: 10.1084/jem.20150489

Figure 8.

Figure 8.

Co-expression of SOX4 rescues the defect in B cell development with expression of miR-132 alone. WT C57BL/6 mice were lethally irradiated and reconstituted with donor bone marrow cells expressing either a control (MIG), a miR-132–overexpressing (MIG-miR-132), a SOX4-overexpressing (MIG-SOX4), or a SOX4- and miR-132–overexpressing (MIG-SOX4-miR-132) retroviral vector (n = 5 mice per group). (A) Validation of miR-132 overexpression in donor HSPCs transduced with the labeled constructs. (B) Validation of SOX4 expression in donor HSPCs transduced with the labeled constructs. (C) Representative spleens from MIG, MIG-miR-132, MIG-SOX4, and MIG-SOX4-miR-132 mice at 4 mo after reconstitution. (D) Select LNs from MIG and MIG-SOX4 mice. Red arrows point to examples of enlarged LNs in MIG-SOX4 mice. (E) Frequency of B cells in LNs of MIG-SOX4 and MIG-SOX4-miR-132 mice with marked B cell expansion. (F) Frequency of myeloid cells in LNs of MIG-SOX4 and MIG-SOX4-miR-132 mice with marked myeloid cell expansion. (G) Frequency of bone marrow B cells in MIG, MIG-miR-132, MIG-SOX4, and MIG-SOX4-miR-132 mice at 4 mo after reconstitution. (H) Hardy fractions from the above cohorts of mice. Data represents the combination of two independent experiments and is represented as mean ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001, Student’s t test.