FIG 1.

Interaction of NXP2/MORC3 with influenza virus polymerase. Cultures of HEK293T cells were infected with the WSN or Victoria (Vic) influenza virus strain at an MOI of 3 PFU/cell or mock infected (Mock). At 6 hpi, total cell extracts were prepared and immunoprecipitated with anti-NXP2/MORC3 or control antibodies. The presence of NXP2/MORC3, PB1, and PA was examined by Western blotting with an antibody specific for NXP2/MORC3 or a mixture of anti-PB1 and anti-PA antibodies. Input, total cell extracts; NXP2, immunoprecipitates obtained with NXP2/MORC3-specific antibodies; Ctrl, immunoprecipitates obtained with unspecific antibodies. The positions of NXP2/MORC3, PB1, PA, and the IgG heavy chain (Ab) are indicated to the left. Changes in electrophoretic mobility for the PB1 and PA proteins are a consequence of the immunoprecipitation process.