FIG 3.

Kinetics of influenza virus multiplication in NXP2/MORC3-downregulated cells. Cultures of A549 cells were transduced with lentiviral vectors expressing an irrelevant short hairpin RNA (shRNA C) or shRNAs targeting the NXP2/MORC3 mRNA (shRNA1 or shRNA2). When the accumulation levels of NXP2/MORC3 were reduced, the cultures were infected with influenza virus WSN at an MOI of 0.001 PFU/cell. (A) Western blot analysis of NXP2/MORC3 at the indicated days post-lentiviral inoculation (dpi), using β-tubulin as a loading control. Serial 2-fold dilutions of control extracts were loaded for improved quantification. (B) The viability of control or downregulated A549 cells was determined using an MTT-based colorimetric assay as described in Materials and Methods. (C) The influenza virus infectivity of the supernatant was determined by plaque assay at the indicated times postinfection. The results presented are representative of three independent experiments. The statistical significance of the differences from the control data set was determined using the Student t test (*, P < 0.05; **, P < 0.01; ***, P < 0.001).