FIG 2.

Two amino acid residues within hCD134 are required for HHV-6B infection. (A) JJHan cells were transduced with nonreplicative lentiviruses expressing hCD134 or its mutants for 24 h. A portion of the transduced cells was incubated with anti-CD134 antibody (10) and secondary antibody (FITC-conjugated rabbit anti-mouse IgG; Dako) at 4°C for 1 h. Then, the cells were subjected to surface expression analysis of hCD134 and its mutants by flow cytometry (EC800; Sony). (B and C) The remaining cells were infected with HHV-6B (HST strain). Cells were harvested at 24 h postinfection. A portion of the cells were fixed with acetone-methanol (7:3) and stained with anti-IE1 antibody and secondary antibody for IFA (B). The remaining cells were lysed and examined by Western blotting with anti-IE1 and anti-α-tubulin antibodies (C). JJHan cells transduced with nonreplicative lentiviruses expressing no alien protein were used as a control for these experiments.